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目的确定菠菜及纯品油胶囊中β-胡萝卜素在学龄儿童体内的转化效率。方法选取某农村小学7～9岁儿童32名,随机分为2组,每组16名,维生素A正常和缺乏者各8名,前7天每天午饭及晚饭两组分别补充氘标记菠菜5g(含2H10-β-胡萝卜素230μg)及纯品β-胡萝卜素油胶囊(含2H8-β-胡萝卜素200μg),早饭两组均服用13C10醋酸视黄醇100μg。在实验的第3、7、8、22及28天分别采集静脉血3ml,用HPLC及GC-MS测定血清中的视黄醇浓度及标记同位素的丰度,计算菠菜及纯品油胶囊中β-胡萝卜素的转化效率。结果β-胡萝卜素油胶囊组维生素A正常和边缘缺乏的儿童β-胡萝卜素的转化率分别为2.9∶1和3.2∶1(质量比);菠菜组分别为10.1∶1和10.3∶1。结论纯品油胶囊中β-胡萝卜素的转化效率明显高于菠菜;不同维生素A营养状况的儿童β-胡萝卜素的转化效率无明显差异。
Abstract：Objective To determine the bioefficacy of pure β-carotene and β-carotene from spinach to vitamin A in schoolchildren of different vitamin A status.Methods Thirty two schoolchildren aged 7-9 years old were selected and then divided into two groups randomly.Each group included 16 subjects,of which the vitamin A level of 8 subjects was normal,the other 8 subjects suffered from vitamin A deficiency.In the first 7 days,one group was given 5 gram spinach(containing 230μg 2H_ 10-β-carotene)before lunch and dinner and the other group was given pure β-carotene capsule(containing 200μg β-carotene).Before breakfast,both groups were given 100μg 13 C_ 10 retinal acetate capsule.Blood was drawn on the 3,7,8,22 and 28 day.HPLC and GC-MS were used to determine the retinol concentration and enrichment of isotope and then calculate the bioefficacy of pure β-carotene and β-carotene from spinach to vitamin A.Results The conversion factors of pure β-carotene to vitamin A in oil capsule were:2.9∶1 for the children with normal vitamin A status,3.2∶1 for those suffering from marginal vitamin A deficiency.The conversion factors of spinach β-carotene to vitamin A were 10.1∶1 and 10.3∶1 for the children with normal and low vitamin A status,respectively.Conclusion The vitamin A conversion bioefficacy of pure β-carotene from oil capsule is much higher than that of spinach.There is no difference of β-carotene bioefficacy to vitamin A for children with different vitamin A status.
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基本信息:
中图分类号:R151
引用信息:
[1]李蕾,王茵,武洁姝,等.学龄儿童体内植物源性胡萝卜素转化成维生素A的效率研究[J].卫生研究,2007(05):547-551.
基金信息:
国家自然科学基金资助项目(No.30571574)
2007-09-30
2007-09-30