卫生研究

样品经粉碎后以乙腈－１％磷酸（９９＋１）提取，过滤后取滤液通过ＰＳＡ固相萃取小柱，洗脱后减压蒸干，用乙腈－水（４＋６）溶解，２０μｌ进色谱柱，荧光检测器检测。结果：咖啡、燕麦、大麦、玉米各一份，花生、黑麦２份检出赭曲霉毒素Ａ。其余样品未检出。本法对大米、玉米、小麦、大麦等回收率均在９０％以上，最低检测限为０．０５μｇ／ｋｇ。结论：使用ＰＳＡ柱可极大简化分析手续，使方法快速、简便、准确，同时不使用对人有害、对环境有污染的有机溶剂，适用于咖啡及各类粮食及制品中赭曲霉毒素的测定，并能实现赭曲霉毒素Ａ、Ｂ的同时测定

おhe powderes samples were extracted with acetonitrile and 1% phosphonic acid (99+1). The filtrate was passed through PSA cartridge and the eluate was evaporated to dryness under low pressure and dissolved in 200μl acetonitrile water (4+6). 20μl of this solution was injected in HPLC. Results:Ochratoxin A was detected in one of the roast coffee, oat, barkey and corn samples and two of the peanut and rye samples. The recoveries were above 90% and the detection limit was 005 μg/kg. Conclusion: The use of PSA cactridge can simpilify the analytical procedure, make the method fast, simple and accurate, while the bodytoxic harmful organic solvents were not used in present method. This method is suitable for coffee, cereals and their products, ochratoxin A and B could be simultaneously determinated according to the described methods.