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目的观察视黄酸受体(RARs)激动剂四氢四甲基萘丙烯苯甲酸乙酯(TTNPB)和类视黄醇X受体(RXRs)激动剂甲氧普烯酸(MA)对脐血单个核细胞表达IL-4和IFN-γ的影响。方法采用ELISA和RT-PCR,分别从蛋白质和基因水平上观察TTNPB和MA对脐血单个核细胞表达IL-4和IFN-γ的影响。结果 ELISA显示10-8、10-6、10-5、10-4mol/L的TTNPB和10-8、10-5、10-4mol/L的MA显著降低IFN-γ/IL-4蛋白含量比值(P<0.05);RT-PCR显示10-4mol/L的MA显著降低IFN-γ/IL-4mRNA比值(P<0.05),而10-5mol/L的TTNPB和10-7、10-6mol/L的MA显著升高IFN-γ/IL-4mRNA比值(P<0.05)。结论适当浓度的TTNPB和MA可以在蛋白质水平上下调IFN-γ/IL-4的比值;不同浓度的TTNPB和MA在基因水平上对IFN-γ/IL-4的比值有不同影响。
Abstract：Objective To observe the effects of retinoic acid receptors agonist TTNPB and retinoid X receptors agonist MA on both protein and gene expression of IFN-γ and IL-4 in cultured cord blood mononuclear cells.Methods Observing both the protein content and gene expression of IFN-γ and IL-4 with ELISA and fluorescent real-time quantitative PCR in vitro.Results It was indicated by ELISA that 10 -8 ,10 -6 ,10 -5 and 10 -4 mol /L TTNPB and 10 -8 ,10 -5 and 10 -4 mol /L MA decreased the ratio of IFN-γ /IL-4 significantly in comparison with the control (P < 0.05 ),and indicated by RT-PCR that 10 -4 mol /L MA decreased the ratio of IFN-γ /IL-4mRNA significantly and 10 -5 mol /L TTNPB and 10 -7 , 10 -6 mol /L MA increased the ratio of IFN-γ /IL-4mRNA significantly in comparison with the control( P < 0.05).Conclusion The ratio of IFN-γ /IL-4 could be down - regulated by proper concentration of TTNPB and MA at the protein level,while the ratio of IFN-γ /IL-4mRNA was differently influenced by various concentration of TTNPB and MA at the gene level.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2010.04.011
中图分类号:R965
引用信息:
[1]朱东洋,王黎荔,李云,等.视黄酸受体和类视黄醇X受体激动剂对脐血单个核细胞IL-4和IFN-γ表达的影响[J].卫生研究,2010,39(04):433-435.DOI:10.19813/j.cnki.weishengyanjiu.2010.04.011.
基金信息:
国家自然科学基金(No.30671761)
2010-07-30
2010-07-30