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目的研究番茄红素对体外培养的雄激素非依赖性前列腺癌细胞PC-3的抑制作用及其可能的作用机制。方法采用MTT法和H3-TdR掺入法观察番茄红素对癌细胞增殖的影响,流式细胞仪观察同步化的细胞经番茄红素作用后细胞周期及凋亡的变化,RT-PCR检测cyclin D1、bcl-2、bax的mRNA的表达的变化。结果番茄红素抑制PC-3细胞的增殖和DNA合成、诱导其凋亡、改变细胞周期分布(使G0/G1期细胞增多、而S期和G2/M期细胞减少)。RT-PCR结果显示,cyclin D1和bcl-2的mRNA表达水平下调,而bax的mRNA表达水平上调。上述作用呈剂量效应关系。结论番茄红素可诱导PC-3细胞凋亡、改变细胞周期分布、影响cyclin D1、bcl-2、bax的mRNA的表达,从而抑制肿瘤细胞增殖。
Abstract：Objective To investigate the effects of lycopene on proliferation of human prostate cancer cell line PC-3 and explore its effecting mechanism.Methods The proliferation of PC-3 cells were analyzed by MTT and H3-TdR incorporation.The effects of cell cycle and apoptosis of synchronized cells were observed through flow cytometry.RT-PCR methods were used to explore the mRNA expression level of cyclin D1,bcl-2,bax.Results The growth and DNA synthesis of PC-3 cell were inhibited with the lycopene concentration increased,and lycopene also could change the cell cycle distribution,i.e.increasing the proportion of G0/G1 phase and descending the proportion of S and G2/M phase,and induce the apoptosis.RT-PCR analysis showed that the mRNA expression level of cyclin D1 and bcl-2 were down regulated,while the level of bax was up regulated.Conclusion Lycopene can induce apoptosis of PC-3,change the cell cycle distribution and downregulate the expression of cyclin D1 and bcl-2 and upregulate the expression of bax and then restrain cell proliferation.
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基本信息:
中图分类号:R737.25
引用信息:
[1]王爱红,张立实.番茄红素对前列腺癌细胞PC-3增殖和细胞周期的影响[J].卫生研究,2007(05):575-578.
2007-09-30
2007-09-30