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目的了解中国市售生畜肉中常见食源性致病菌的污染状况。方法2014年在中国15个省(自治区、直辖市)采集生畜肉4308份,按照食品安全国家标准方法检测金黄色葡萄球菌、单核细胞增生李斯特菌和沙门菌。结果沙门菌检出率为7.50%(323/4308),猪肉检出率高于牛羊肉,血清型主要是德尔卑沙门菌和鼠伤寒沙门菌。单核细胞增生李斯特菌的检出率为8.84%(381/4308),超市和餐饮店的检出率高于农贸市场,冻畜肉和冷却畜肉的检出率高于鲜畜肉。金黄色葡萄球菌的检出率为12.21%(526/4308),94.30%(494/526)的阳性样品≤10~3CFU/g。3种致病菌在第二、三季度的检出率均高于第一、四季度。结论中国生畜肉中致病菌检出率较高,通过交叉污染导致食源性疾病的可能性较大,需加以重视。
Abstract：[1]雷质文.肉及肉制品微生物监测应用手册[M].北京:中国标准出版社,2008:2-9.
[2]MEYER C,FREDRIKSSON-AHOMAA M,SPERNER B,et al.Detection of Listeria monocytogenes in pork and beef using the VIDASLMO2 automated enzyme linked immunoassay method[J].Meat Sci,2011,88(3):594-596.
[3]刘秀梅.我国食品卫生微生物学30年来的热点研究及主要进展[J].中国食品卫生杂志,2009,21(4):289-295.
[4]郭新春,苗志红.一起典型沙门菌污染牛肉引起食物中毒的分析[J].实用医技杂志,2001,8(8):622.
[5]WU Yongning,LIU Xiumei,CHEN Qian,et al.Surveillance for foodborne disease outbreaks in China,2003 to 2008[J].Food Control,2018,84:382-388.
[6]USDA,FSIS.Progress report on Salmonella and Campylobacter testing of raw meat and poultry products,1998-2014[R/OL].[2017-10-01].https://www.fsis.usda.gov/wps/wcm/connect/fsiscontent/internet/main/topics/data-collection-andreports/microbiology/annual-progress-reports.
[7]European Food Safety Authority,European Centre for Disease Prevention and Control.The European union summary report on trends and sources of zoonoses,zoonotic agents and food-borne outbreaks in 2015[J].EFSA J,2016,14(12):4634.
[8]巢国祥,焦新安,周丽萍,等.食源性金黄色葡萄球菌流行特征、产肠毒素特性及耐药性研究[J].中国卫生检验杂志,2006,(16)8:904-908.
[9]吴平芳,贺连华,石晓路,等.深圳市生肉食品6种食源性致病菌污染状况调查[J].热带医学杂志,2015,15(7):989-991.
[10]杨瑞军,金莞尔,张建民,等.生肉食品中致病菌检测结果分析[J].中国卫生检验杂志,2009,19(9):2122-2123.
[11]United States Department of Agriculture,Food Safety and Inspection Service.Pathogen Reduction/HACCP&HACCP Systems:Final Rule,Docket No.93-016F[S].Federal Register,1996,61(144),25:38805-38989.
[12]European Commission.Commission regulation(EC)No 1441/2007 of 5 December 2007 amending Regulation(EC)No 2073/2005 on microbiological criteria for foodstuffs(Text with EEA Relevance)[S].Official J European Union,2007.
[13]刘以琨,谭艾娟,吕世明,等.贵阳市市售猪肉中食源性致病菌分离鉴定[J].天津农业科学,2012,18(2):72-74.
[14]United States Department of Agriculture,Food Safety and Inspection Service.Serotypes profile of Salmonella isolates from meat and poultry products January 1998 through December 2013[R/OL].[2017-10-01].https://www.fsis.usda.gov/wps/wcm/connect/3866026a-582d-4f0e-a8ce-851b39c7390f/Salmonella-Serotype-Annual-2014.pdf?MOD=AJPERES.
[15]PATCHANEE P,TANSIRICHAROENKUL K,BUAWIRATLERT T,et al.Salmonella in pork retail outlets and dissemination of its pulsotypes through pig production chain in Chiang Mai and surrounding areas,Thailand[J].Prev Vet Med,2016,130:99-105.
[16]周丽萍,巢国祥.生肉制品中沙门菌、单核细胞增生李斯特菌及金黄色葡萄球菌流行特征研究[J].中国卫生检验杂志,2007,17(3):505-507.
[17]CHURCHILL R L,LEE H,HALL J C.Detection of Listeria monocytogenes and the toxin listeriolysin O in food[J].J Microbiol Methods,2006,64(2):141-170.
[18]FRUSTOLI M A,GRISENTI M S,LORI D,et al.Determinaziton of Listeria monocytogenes in fresh pork meat:Validation of a rapid method[J].Industria Conserve,2005,80(5):285-292.
[19]WIECZOREK K,DMOWSKA K,OSEK J.Characterization and antimicrobial resistance of Listeria monocytogenes isolated from retail beef meat in Poland[J].Foodborne Pathog Dis,2012,9(8):681-685.
[20]高涛.食品中金黄色葡萄球菌肠毒素及检测方法的研究进展[J].福建分析测试,2003,1(2):1775-1778.
[21]国家卫生计生委办公厅关于2015年全国食物中毒事件情况的通报[EB/OL].[2017-10-01].http://www.nhfpc.gov.cn/yjb/s7859/201604/8d34e4c442c54d33909319954c43311c.shtml.
[22]U.S.Food and Drug Administration.Bad bug book:foodborne pathogenic microorganisms and natural toxins handbook Staphylococcus aureus[R/OL].[2017-10-01].http://pdf.usaid.gov/pdf_docs/Pnado152.pdf.
[23]ILBOUDO A J,TAPSOBA F,SAVADOGO A,et al.Improvement of the hygienic quality of farmhouse meat pies produced in Burkina Faso[J].Advanc Environ Biol,2012,6(10):2627-2635.
基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2018.03.028
中图分类号:TS251.7
引用信息:
[1]闫琳,裴晓燕,宋筱瑜,等.2014年中国15省(自治区、直辖市)市售生畜肉中常见食源性致病菌污染状况研究[J].卫生研究,2018,47(03):498-501+511.DOI:10.19813/j.cnki.weishengyanjiu.2018.03.028.
基金信息:
国家重点研发计划(No.2017YFC1601503); 国家食品安全风险评估中心高层次人才队伍建设523项目
2018-05-25
2018-05-25