卫生研究

目的探讨不同浓度乙醇对果蝇寿命以及果蝇体内超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力、以及丙二醛(MDA)含量的影响。方法收集8小时内羽化未交配雌雄果蝇7200只,雌雄各半。取其中2400只,随机分为6组,每组雌雄果蝇各200只,给予不同浓度乙醇染毒,每4天更换1次培养基,每天记录死亡数,用生存实验检测乙醇对果蝇寿命的影响。另取4800只,随机分为4组,每组雌雄各600只,给予含不同浓度乙醇的培养基喂养,分别于喂养0天、10天、20天和30天测定SOD和GSH-Px活力,同时检测MDA的含量。结果随着乙醇剂量的增加,雌雄果蝇的寿命均呈下降趋势,高剂量乙醇染毒组果蝇寿命显著降低(P<0.05)。在相同染毒剂量下,随着染毒时间的增加,果蝇体内SOD、GSH-Px活性逐渐降低,MDA含量逐渐升高;当染毒20天和30天时,90mmol/L、270mmol/L剂量组SOD、GSH-Px活性及MDA含量与对照组相比差异有显著性(P<0.05)。结论乙醇暴露能降低果蝇寿命,并导致果蝇体内SOD和GSH-Px活力的降低和MDA含量的增高。

Objective To explore the effect of ethanol on the life span and the activities of SOD and GSH-Px as well as the contents of MDA in drosophila melanogaster. Methods Newly trapped and not mated 7200 flies were collected within 8 hours and separated by sex. 2400 flies were selected and randomly divided into six groups (0, 9, 27, 90, 270 and 810mmol/L). Each group contained 400 flies, 200 male flies and 200 female flies. The numbers of dead flies were counted everyday and the culture mediums were replaced every 4 days until all flies died. Another 4800 flies were randomly divided into 4 groups (0, 27, 90 and 270mmol/L). Each group contained 1200 flies, half male and half female. The activities of SOD and GSH-Px as well as the contents of MDA were detected at the days of 0th, 10th, 20th and 30th after ethanol exposure. Results The life span of drosophila exposed to high dose ethanol reduced obviously. Activities of SOD and GSH-Px and contents of MDA of drosophila exposed to ethanol reduced and enhanced with increasing of ethanol doses. And at the same doses (90mmol/L and 270mmol/L), activities of SOD and GSH-Px reduced, while contents of MDA elevated with increasing of exposure time. Exposed to high dose of ethanol at the days of 20 or 30, activities of SOD and GSH-Px and contents of MDA in drosophila reduced and enhanced significantly in comparison with control group. Conclusion Ethanol could reduce the life span and the activities of SOD and GSH-Px , and enhance MDA content of drosophila.