卫生研究

目的采用免疫功能低下小鼠模型探讨灵芝孢子油免疫调节作用机制。方法将昆明小鼠随机分成模型对照组、灵芝孢子油组、正常对照组,每组10只;模型对照组、灵芝孢子油组分别腹腔注射40mg.kg-1.-1)环磷酰胺建立免疫功能低下小鼠模型,正常对照组腹腔注射生理盐水(0.9%)0.1ml/10g BW;同时灵芝孢子油组150mg/kg,正常和模型对照组灌胃玉米油0.1ml/10g BW,灌胃1次/A,连续30d;用ELISA试剂盒检测小鼠血清TNF-α、IFN-水平,半定量PCR检测小鼠脾脏、胸腺IL-2、IL-10、IL-12、IL-4、IFN-、TNF-α基因表达量,初步探讨灵芝孢子油的免疫调节机制。结果灵芝孢子油明显提高环磷酰胺免疫低下模型小鼠血清中TNF-α、IFN-的含量,脾脏和胸腺中IL-2、IL-10、IL-12、IL-4、TNF-α和IFN-mRNA的表达量,与模型对照组间差异显著(P≤0.05)。结论灵芝孢子油通过增强细胞因子的分泌、表达进行免疫调节。

Objective To study the rudimentary immunoregulatory mechanisms of Ganoderma spore oil on immunocompromized mice model.Methods Thrity KM mice were randomly selected and assigned into three groups(ten animals per group):the model control group,Ganoderma Lucidum spores oil group and the normal control group.The model control group and Ganoderma Lucidum spores oil group were injected intraperitoneally with cyclophosphamide at 40mg·kg-1·d-1 to generate a immunocompromized mice model.The normal control group were administered with 0.9% NaCl solution 0.1ml/10g BW as placebo.All agents were given orally once a day,given for consecutive 30 days,Ganoderma Lucidum spores oil group 150mg/kg,the others given maize 0.1ml/10g BW.The serum TNF-α,IFN- content of the mice through ELISA kit and the expression levels of IL-2,IL-10,IL-12,IL-4,IFN-,TNF-α mRNA in mouse spleen and thymus were examined by RT-PCR to rudimentary study its immunoregulatory mechanisms.Results Ganoderma spore oil can significantly increased the content of TNF-α and IFN- in the serum and the expression levels of IL-2,IL-10,IL-12,IL-4,IFN-,TNF-α mRNA in spleen and thymus,with obvious difference from the model control(P≤0.05).Conclusion Ganoderma spore oil can be able to improve the above cytokineion expression to immunoregulate the immunocompromized mice.