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目的探索甲醛对脱细胞DNA的影响,并初步建立脱细胞-核DNA检测加合物的新模型。方法用染毒缓冲液配制浓度分别为4%、1%、0.25%和0%的甲醛,用羟自由基损伤的脱细胞-核DNA作为检测加合物的试验模型,每组6张脱细胞-核DNA板,用彗星实验检测各组脱细胞-核DNA损伤情况,组间差异用SPSS 11.0软件统计分析。结果甲醛染毒的DNA损伤顺序为:0%=0.25%>1%组>4%,呈现明显的剂量-反应关系。结论甲醛可直接和脱细胞DNA片段形成加合物和/或DNA-DNA交联,脱细胞-核DNA模型可用于DNA加合物和/或交联物的检测。
Abstract：Objective To explore the impact of formaldehyde on acellular-nuclear DNA,and initially to establish a new model of acellular nuclear DNA to test adducts.Methods The acellular-nuclear DNA used as a test model,sealed boxes pre-positioned slides fixed with acellular-nuclear DNA.Different concentrations(4%,1%,0.25% and 0%)of formaldehyde were added to boxes.Six acellular-nuclear DNA slides were used in each group.DNA damage was measured with comet assay,and statistical analysis of the difference between the groups with SPSS 11.0 software.Results DNA damage exposed to formaldehyde in such sequences: 0%=0.25%>1%>4% with significant dose-response relationship.Conclusion Formaldehyde can directly react with acellular DNA,and form adducts and/or DNA-DNA crosslinking,acellular-Nuclear DNA model can be used to detect DNA adducts and cross-linked.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2013.01.034
中图分类号:R114
引用信息:
[1]杨丽娟,王越越,朱海燕.甲醛对脱细胞DNA的影响[J].卫生研究,2013,42(01):143-146.DOI:10.19813/j.cnki.weishengyanjiu.2013.01.034.
基金信息:
国家自然科学基金资助项目(No.30900543)
2013-01-30
2013-01-30