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目的探讨DNA甲基化转移酶3a基因(DNMT3a)的单核苷酸多态性与中国汉族噪声暴露工人的噪声性听力损失(NIHL)易感性之间的关联。方法采用病例-对照的研究方法,以江苏省某化纤工厂和某能源公司2015年参加职业健康体检的噪声暴露的998名作业工人(男性908人,女性90人)为研究对象。通过问卷调查和现场噪声检测(GBZ/T 189.8—2007)获取研究对象的一般信息和噪声暴露情况,按照纯音气导听阈测试(GBZ 49—2014)的结果将其分为病例组(n=498,双耳高频平均听阈>25 dB)和人为匹配的对照组(n=500,双耳高频平均听阈≤25 dB)。采用TaqMan探针法对DNMT3a的单核苷酸多态性位点rs7590760进行基因分型。结果显性模型中,rs7590760各基因型在病例组和对照组之间的分布差异具有统计学意义(P=0.001),运用Logistics回归模型对性别、年龄、吸烟和饮酒情况进行调整后,CC/CG基因型噪声性听力损失发病风险是GG基因型发病风险的1.56倍,调整OR=1.56(95%CI 1.222.01)。对接噪工龄和噪声暴露水平进行分层分析后发现,当噪声作业工龄≤16年和>16年时,调整OR分别为1.67(95%CI 1.172.38)和1.52(95%CI 1.062.17);在≤85 dB、86~92 dB和>92 dB 3种噪声暴露强度下,对应的调整OR分别为1.56(95%CI 1.062.30)、1.67(95%CI 0.942.99)和1.51(95%CI 1.012.26)。结论 DNMT3a基因rs7590760位点的CC/CG基因型可能是中国汉族噪声暴露作业工人发生噪声性听力损失的危险因素。
Abstract：Objective To investigate the potential association of DNMT3a's single nucleotide polymorphism with susceptibility to noise-induced hearing loss( NIHL) in Chinese noise-exposed workers.Methods A case-control study was performed,and 998 noise-exposed workers from a chemical fibre factory and an energy company, who underwent occupational health examination in 2015,were enrolled as study subjects.Then,general information and noise exposure of the study subjects were obtained through questionnaire survey and on-site noise detection.According to the result of audiological evaluation,they were divided into case group( n = 498,high-frequency threshold shift >25 dB) and man-matched control group( n = 500,high-frequency threshold shift ≤25 dB).At last,genotyping of DNMT3a rs7590760 was conducted with TaqMan-PCR technique.Results In the dominant model,the distribution of rs7590760 genotypes between the case group and the control group was statistically significant( P = 0.001).The NIHL risk of subjects with CC/CG genotype is 1.56 times the risk of those carrying GG genotype,with an adjusted OR = 1.56( 95% CI 1.22-2.01).After the noise exposure period and noise exposure intensity were stratified,the adjusted OR values for noise exposure period ≤16 years, > 16 years and noise intensity ≤85,86-92 and >92 dB were respectively 1.67( 95% CI 1.17-2.38),1.52( 95% CI 1.06-2.17),1.56( 95% CI 1.06-2.30),1.67( 95% CI 0.94-2.99) and 1.51( 95% CI 1.01-2.26).Conclusion The CC/CG genotype of rs7590760 in DNMT3a gene is a potential risk factor for noise-induced hearing loss in Chinese noise-exposed workers.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2018.04.024
中图分类号:R135
引用信息:
[1]郭浩然,程剑,盛荣建,等.DNMT3a单核苷酸多态性与噪声性听力损失易感性的关联研究[J].卫生研究,2018,47(04):638-643.DOI:10.19813/j.cnki.weishengyanjiu.2018.04.024.
基金信息:
江苏省医学创新团队(No.CXTDA2017029)
2018-01-05
2018
2018-05-07
2024-06-18
2024
7
2018-07-27
2018-07-27