| 202 | 8 | 14 |
| 下载次数 | 被引频次 | 阅读次数 |
目的研究镓盐对维甲酸致骨质疏松大鼠骨代谢的影响。方法通过维甲酸灌胃给药建立大鼠骨质疏松模型,观察血、尿及骨的代谢变化;建立骨质疏松模型后,实验分为四组:正常对照组、骨质疏松组、雌激素治疗组和氯化镓治疗组,治疗两个月,观察治疗前后模型动物骨代谢及血清和尿中相关指标的影响。同时,动态比较治疗30天和治疗60天的治疗效果。结果维甲酸导致大鼠骨结构改变,密度降低,骨有机基质含量下降,血清抗酒石酸酸性磷酸酶上升。应用氯化镓后,可减轻因维甲酸激活破骨细胞使骨吸收增强造成的上述损伤,大鼠平均骨小梁宽度、平均骨皮质厚度、骨小梁百分比增加;TRAP和AKP活性降低,接近正常组水平。结论镓盐是一种有效的降低骨转化率的药物。
Abstract：Objective To investigate the effect of gallium salts on bone metabolism in osteoporosis rats caused by tretinoin. Methods After duplicating osteoporotic model of rats by using tretinoin, we observed the metabolism of blood,urine and bone in vivo.The rats were divided into control group, osteoporosis group, estrogen treated and gallium chloride treated group. The indexes of bone metabolism and related indexes in serum and urine were observed after 60 days of treatment. In the same time, we observed the dynamic effect of 30 days and 60 days of treatment. Rtesults After duplicating osteoporotic model,rats' bone structures were injured, the bone mineral density and the organic matrix decreased, the contents of tartrate-resistant acid phosphatase(TRAP) in serum were higher. After gallium salt treating, the above damages were inhibited or retarded. Trabecular width and thickness of bone cortex were significantly increased. AKP and TRAP activities were decreased to the level close to that of the control group. Conclusion Gallium salt is effective in treating osteoporosis.
1刘忠厚,杨定焯,朱汉民,等.中国人原发性骨质疏松症诊断标准(试行).中国骨质疏松杂志,1999,5(1):1
2张矢远,莫晓燕.软骨生化与骨软骨疾病.陕西科学技术出版社.1996,206-215
3Lau K H W,Onishi T,Wergedel JE,et al.Characterigation and assay of tartrate resistant acid phosphatase activity in serum.Clin chem,1987,33(4):458
4Schapira D,Laton-Miller R,Barzilai D,et al.The animal as a model for studies of the aging skeleton.Cells Mater,1992,(Suppl)1:181
5王大申,张梅.骨钙素与骨代谢.国外医学内分泌分册,1985,(4):180
6David MS,John SA,Robert MN,et al.Deficient production of1α,25-dihydroxy vitamin Din elderly osteoporosis patients.NEngl J Med,1981,1:305
7Delmas PD.Biochemical marker of boneturnoverforthe clinical assesment of metabolic bone disease.Endocrinol Metab Clin North Am,1990,19:1
8Shevde NK.Estrogen modulates the recruitment of myelopoietic cell progenitorsin rat through a stromal cell-independent mechanisminvolving apoptosis.Blood,1996,87:2683
9Warrell RP.Effects of galliumon bone in the rats.J Bone Miner Res,1992,71:11
基本信息:
中图分类号:R96
引用信息:
[1]代晓霞,熊咏民,郭雄,等.镓盐对维甲酸致大鼠骨质疏松模型影响的动态实验研究[J].卫生研究,2006(02):175-178.
基金信息:
陕西省自然科学基金项目资助(2003K03G1)
2006-03-30
2006-03-30