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采用~3H-TdR掺入实验观察了D,L-硒代蛋氨酸与亚硒酸钠对猪肾近曲小管上皮细胞(LLC-PK1)DNA合成代谢的影响。结果显示,两种硒化合物对DNA的合成均呈双相效应,在硒浓度为0.5～50μmol/L时,能促进DNA的合成,并且D,L-硒代蛋氨酸的作用比亚硒酸钠更为有效;随硒浓度的增高,两种硒化合物均呈现对LLC-PK1细胞DNA合成的抑制作用,亚硒酸钠的毒性作用明显大于D,L-硒代蛋氨酸,亚硒酸钠浓度为400μmol/L时,LLC-PK1细胞DNA合成的抑制率为94.1%,而D,L-硒代蛋氨酸浓度为400μmol/L时,仅呈现对LLC-PK1细胞DNA合成的轻度抑制作用,与文献结果比较,LLC-PK1细胞对硒的毒性作用有较高的耐受性。
Abstract：The effects of selenomethionine(SeMet) and sodium selenite(Na_2SeO3) on DNA synthesis were studied in pig kidney epithelial cell line (LLC-PK1). Results revealed a biphasic effects of both Na_2SeO3 and SeMet on DNA synthesis. At concentrations between 0.5 and 50μmol/L, both of selenium compounds stimulated cell DNA synthesis and SeMet was more effective. At concentrations of 200μmol/L and above they inhibited cell DNA synthesis, and the toxicity of Na_2SeO3 was greater than SeMet.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.1996.01.018
中图分类号:R151
引用信息:
[1]彭双清,刘世杰.硒代蛋氨酸与亚硒酸钠对LLC-PK_1细胞DNA合成影响的比较[J].卫生研究,1996(01):50-52.DOI:10.19813/j.cnki.weishengyanjiu.1996.01.018.
基金信息:
国家自然科学基金
1996-01-20
1996-01-20