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目的 探讨西南喀斯特地区农村典型水源水中耐药基因(antibiotic resistance genes, ARGs)的分布及其与细菌群落的关联性。方法 于2022年10月—2023年10月使用无菌采样袋采集贵州省9个市(州)喀斯特地区农村典型饮用水源水(窖水、山泉水、井水、地表水)共36份。分别采用16S rRNA高通量测序技术和PCR法分析水源水中细菌群落成分以及检测磺胺类(sul1、sul2、sul3)、喹诺酮类(qnrB、qnrS)、大环内脂类(ermB、erm36)及四环素类(tetA、tetO、tetM)共四类10种常见ARGs的分布，采用列联表卡方检验分析ARGs与细菌群落的关联性。结果 36份水样中检出鞘氨醇单胞菌属(Sphingomonas)等50个菌属，其中有8种潜在病原菌属，分别为黄杆菌属(Flavobacterium)、假单胞菌属(Pseudomonas)、不动杆菌属(Acinetobacter)、鞘氨醇单胞菌属(Sphingomonas)、短波单胞菌属(Brevundimonas)、蛭弧菌属(Bdellovibrio)、军团菌属(Legionella)、葡萄球菌属(Staphylococcus)。四类10种ARGs在36个水样中的总检出率为38.89%,在窖水、山泉水、井水、地表水中的总检出率分别为22.00%、42.50%、44.44%、54.00%;磺胺类、喹诺酮类、大环内脂类及四环素类ARGs在36个水样中的平均检出率分别为47.22%、18.06%、43.06%、41.67%,其中sul1、sul2、ermB、tetO检出率较高，分别为63.88%、58.33%、58.33%、55.56%;四类ARGs在地表水中检出率较高，分别为60.00%、30.00%、60.00%、60.00%;各ARGs至少有一个潜在宿主菌，其中erm36存在最多的潜在宿主菌，其与草螺菌属(Herbaspirillum)、Chloroplast、动胶杆菌属(Zoogloea)、柄杆菌属(Caulobacter)存在关联(r=0.018、0.001、0.019、0.046)。结论 西南喀斯特地区农村典型水源水中ARGs检出较高，各ARGs均有至少一个潜在宿主菌，对人群健康存在潜在风险。
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2024.06.027
中图分类号:R123.1
引用信息:
[1]张凤兰,陈刚,谢春,等.西南喀斯特地区农村典型水源水耐药基因分布[J].卫生研究,2024,53(06):1021-1026.DOI:10.19813/j.cnki.weishengyanjiu.2024.06.027.
基金信息:
贵州省科技支撑计划(黔科合支撑[2021]一般024)
2024-11-30
2024-11-30