卫生研究

目的尝试探讨正常大鼠血糖分布范围并进行影响因素效应评估。方法收集不同实验中近500只正常Wistar或SD大鼠空腹血糖(FBG)和餐后120min血糖(PBG)数据,采用多变量方差分析和分类变量最优尺度回归分析血糖分布范围并筛选关键影响因素。另选用40～100只Wistar大鼠,采用组间对照或自身对照实验设计,比较葡萄糖氧化酶法与血糖监测仪法间、禁食5h与11h间、腹主动脉血与尾血间、采血后不同处理时间血糖结果的差异;建立血糖测量误差评价的数学模型,以半宽或极差对各影响分量的贡献进行评估并合成。结果Wistar大鼠血糖正常值为FBG(3.95±1.31)mmol/L,PBG(5.65±1.63)mmol/L,95%上限分别为6.2mmol/L和7.9mmol/L。大鼠品种和采血部位对血糖分布没有明显影响,血糖在7～10mmol/L范围外血糖监测仪法与葡萄糖氧化酶法不具可比性,禁食5h时的血糖比11h时高0.8mmol/L,腹主动脉血的血糖值高于尾血40%,血样采集后60min内分离血清的血糖衰减率为8%,120min时衰减率达50%(P<0.05)。在重新规定血糖测定操作要求后,确定正常FBG和PBG的95%扩展上限为7.5mmol/L和10.4mmol/L。结论当血糖超过扩展上限时可以判断为糖尿病发生,此结果与人类判断标准接近。

Objective To evaluate the distribution and affecting factors of blood glucose in normal rats for the criterion of hyperglycemia in animal model. Methods The data of fast blood glucose ( FBG ) and 120 min postprandial blood glucose (PBG) of nearly 500 Wistar and SD rats from different experiment in our laboratory were collected and analyzed by multi - variable analysis and optimal scaling regression for the distribution and key affecting factors of blood glucose. Comparing the blood glucose determined by different method ( glucose oxidase method or glucose monitor with strips),different duration ( fasting for 5h or 11h),different blood source ( from abdominal aorta or tail),and different time for separating serum after sampling ( at once or till 120min) was conducted in another 40 - 100 Wistar rats. A mathematical model for the contribution of different components was evaluated and combined by parameters of half width and range. Results The average FBG and PBG of normal Wistar rats was (3. 95 ± 1. 31) mmol /L and (5. 65 ± 1. 63)mmol /L,and the 95% upper limit was 6. 2mmol /L and 7. 9mmol /L respectively. The differences of fasting duration,sample source,time before separating serum and testing methods,excepting for rat species,were significant (P < 0. 05). The results showed that the blood glucose tested by glucose monitor method was not comparable with GOD method while the range of blood glucose was out of 7 - 11mmol /L. The difference of blood glucose between fasting for 5h and 11h was 0. 8mmol /L. Blood glucose from abdominal aorta was 40% higher than that from tail. The reduction of blood glucose was 8% in 60min and over 50% in 120min after blood being sampled. After the test requirement being regulated,the upper limit for FBG and PBG was expanded to 7. 5mmol /L and 10. 4 mmol /L,respectively. Conclusion The onset of diabetics in rat is judged as blood glucose being higher than the expanded normal upper level. The criteria for rats are close to that for human.