| 128 | 30 | 26 |
| 下载次数 | 被引频次 | 阅读次数 |
为研究氟对大鼠大脑皮层神经细胞的 DNA损伤作用及硒、锌单独及联合作用对其的影响 ,对大鼠体外染毒后采用单细胞凝胶电泳技术检测 DNA单链断裂。结果显示单独加氟、硒组 DNA损伤程度显著大于对照组 (P<0 .0 1) ,且氟的影响更为严重 ,氟硒组和氟锌组 DNA损伤程度小于氟组 ,但差异无显著性意义 ,氟硒锌组 DNA损伤程度显著低于氟组 (P<0 .0 5 )。提示氟、硒单独作用均造成大脑皮层神经细胞 DNA损伤 ,硒锌联合可明显拮抗氟对 DNA的损伤作用。
Abstract：To investigate the effects of fluoride on DNA damage as well as the effects of selenium and zinc against fluoride respectively or jointly in pallium neural cells of rats ,single cell gel electrophoresis was used to detectthe DNA dam age of neural cells prepared in vitro.The results showed that the degree of DNA dam age in the fluoride group and the selenium group were significantly greater than that in control group(P <0 .0 1) .The damage in the fluoride group was even more serious.The damage in the fluoride+ selenium group and fluoride + zinc group was slighter than that in the fluoride group but with no significant difference.The extent of DNA dam age in the fluoride+ selenium+ zinc group was significantly slighter than that in the fluoride group(P<0 .0 5 ) .It suggested that fluoride and selenium could induce DNA damage in pallium neural cells of rats respectively.Moreover,the joint antagonistic effect of selenium and zinc against fluoride was more obvious.
1 张本忠 ,吴德生 .无机氟对大小鼠胚胎中脑细胞增殖和分化的影响 .中国地方病学杂志 ,1998,13(3) :12 9— 131
2 独立 .慢性氟中毒对人胎脑发育的影响 .中华病理学杂志 ,1992 ,2 1(4) :5 8— 71
3 马道新 ,陈正岗 ,刘春生 ,等 .氟化物遗传毒性的分子生物学研究进展 .国外医学医学地理学分册 ,1999,2 0 (1) :1— 9
4 Dildy JE,L eslie SW.Ethanol inhibits NMDA-induced increase infree intracellular Ca2 + in dissociated brain cells.Brain Res,1989,499(31) :383— 389
5 Singh NP,Mccoy MT.A simple technique for quantitation of lowlevels of DNA damage in individual cells. Exp Cell Res,1988,175 :184— 189
6 Garberg P,Stahl A,Warholm M,et al.Studies of the role DNAfragmentation in selenium toxicity.Biochem Pharmacol,1988,37:340 1— 340 6
7 Lu JX,Kaeck M,Jiang C,et al.Selenium induction of DNA strandbreaks and apoptosis in mouse leukemic L12 10 cells.BiochemPharmacol,1994,47(9) :15 31— 15 35
8 孙淑庄 ,修瑞琴 ,邓海 ,等 .氟硒联合毒性作用的研究 .卫生研究 ,1996 ,2 5 (1) :2 0— 2 3
9 岑小波 ,王瑞淑 .锌在细胞凋亡中的调控作用及其分子机制 .国外医学卫生学分册 ,1999,2 6 (1) :2 2— 2 5
基本信息:
中图分类号:R114
引用信息:
[1]陈军,陈学敏,杨克敌.硒、锌对氟致大鼠大脑神经细胞DNA损伤的影响[J].卫生研究,2000(04):216-217.
基金信息:
“九·五”国家医学科技攻关!项目 (No.96 -90 6 -0 4-0 2
2000-07-20
2000-07-20