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目的 分析中国十五省(自治区、直辖市)成年居民不同炎症水平下的肠道菌群特征及差异菌属，并分析其与血脂水平的关联。方法 数据选取参与了2018年“中国健康与营养调查”,采集了生物样品并完成全部调查的成年居民共4158人，对血清样品进行生化检测及粪便样品16S扩增子测序，依据超敏C反应蛋白分将人群为低炎症组和高炎症组，通过多样性分析组间菌群物种均匀度和丰富度以及两组人群菌群整体分布特征差异，并采用线性判别分析组间差异菌群，最后将组间的差异菌群与人群的生理生化指标进行关联性分析。结果 高炎症组与低炎症组相比菌群多样性较低，组间整体菌群差异有统计学意义(R2=0.25%,P=0.01),有6种菌属在低炎症组样本中富集，有4种菌属在高炎症组中富集。其中乳酸杆菌属和链球菌属与体质指数正相关，罗姆布茨菌与胆固醇，低密度脂蛋白胆固醇和高密度脂蛋白胆固醇均呈正相关；克氏菌UCG-014,瘤胃球菌属和粪杆菌属与甘油三酯正相关。结论 炎症水平不同的人群肠道菌群整体结构及优势菌存在差异，炎症反应会通过这些优势菌属进而对血脂水平产生一定的影响。
Abstract：OBJECTIVE To analyze the characteristics and differences of gut microbiota in adult residents of 15 provinces of China under different inflammation levels, and to analyze their correlation with blood lipid levels.METHODS A total of 4158 adult residents who participated in the 2018 China Health and Nutrition Survey and completed all the surveys were selected. Biochemical tests were performed on serum samples, and 16S amplicon sequencing was carried out on fecal samples. According to the ultra-sensitive C-reactive protein, the population was divided into a low inflammation group and a high inflammation group. The uniformity and richness of bacterial species and the overall distribution characteristics of bacterial communities between the two groups were analyzed through diversity analysis, and the differences in bacterial communities between the two groups were analyzed using linear discriminant analysis. Finally, the differences in bacterial communities between the groups were analyzed in association with the physiological and biochemical indicators of the population.RESULTS Compared with the low inflammation group, the bacterial diversity in the high inflammation group was lower, and there was a significant difference in the overall bacterial composition between the two groups(R2=0.25%, P=0.01). Six genera were enriched in the samples of the low inflammation group, and four genera were enriched in the high inflammation group. Among them, Lactobacillus and Streptococcus were positively correlated with BMI, and Romboutsia was positively correlated with TC, LDL-C and HDL-C. Clostridium UCG-014, Ruminococcus and Faecalibacterium were positively correlated with TG.CONCLUSIONThe study found that there are differences in the overall structure and dominant bacteria of the gut microbiome in people with different inflammation levels, and the inflammatory response may have a certain impact on blood lipid levels through these dominant bacterial genera.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2025.05.001
中图分类号:R195
引用信息:
[1]关方旭,黄绯绯,张晓帆等.2018年中国十五省(自治区、直辖市)成年居民炎症水平与肠道菌群及血脂的关联[J].卫生研究,2025,54(05):707-714.DOI:10.19813/j.cnki.weishengyanjiu.2025.05.001.
基金信息:
国家重点研发计划(No.2021YFE0114200)