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目的研究生物活性物质体外抗氧化能力评价方法,应用体外理化环境下建立起来的评价方法对受试物进行初步抗氧化能力评估。方法联合应用2,2’-连氮-双-(3-乙基苯并噻唑-6-磺酸)二铵盐(ABTS)法与铁离子还原/抗氧化能力测定法(FRAP)法用于生物活性物质体外抗氧化能力研究,在ABTS法中,使用紫外可见分光光度计734nm波长下测定以槲皮素、姜黄素、DL-α-生育酚和原花青素等为代表的生物活性物质;在FRAP法中,运用酶标仪,595nm波长处测定同样受试物的抗氧化能力。结果ABTS法:槲皮素和姜黄素的抗氧化活性TEAC值分别约为2.02和0.50;而1gDL-α-生育酚、原花青素清除自由基的能力相当于2.06mmol、2.897mmol的Trolox清除自由基的能力;FRAP法:抗氧化活性以1.0mmol/L FeSO4为参考标准,槲皮素、姜黄素和Trolox摩尔当量约为5.73、1.18和2.09;而DL-α-生育酚和原花青素抗氧化活性分别是207.7mg、156.36mg。结论ABTS法与FRAP法联合应用,操作简便、结果可靠,尤其适宜作为生物活性物质体外抗氧化能力的评价方法。
Abstract：Objective To study the evaluation methods for antioxidant capacity of bioactive substance in vitro, and to apply the methods which is established in physicochemical environment in order to give the preliminary assessment of test subjects.Methods The combined application of ABTS and FRAP methods in the assessments, using the UV-VIS spectrophotometer and the autoanalyzer respectively test the antioxidant capacity of quercetin, curcumin, DL-α-tocopherol and procyanidine at 734nm in ABTS and at 595nm in FRAP.Results ABTS: The TEAC values of quercetin and curcumin were about 2.02 and 0.50.1g DL-α-tocopherol and anthocyanins were equivalent to 2.06mmol,2.897mmol of Trolox in scavenging free radicals capacity. FRAP: Used 1.0mmol/L FeSO4 as the reference standard, quercetin, curcumin and Trolox equivalent molar about 5.73,1.18 and 2.09. DL-α-tocopherol, antioxidant activity of proanthocyanidins were 207.7mg and 156.36mg.Conclusion It was supported support that combined ABTS and FRAP methods, because of their conveniently, and their reliable results, especially in appropriately be used as the evaluation methods for antioxidant capacity of bioactive substance in vitro.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2009.03.008
中图分类号:R151.2
引用信息:
[1]刘小兵,朴建华,田园.几种生物活性物质体外抗氧化能力评价技术的研究[J].卫生研究,2009,38(03):280-283.DOI:10.19813/j.cnki.weishengyanjiu.2009.03.008.
基金信息:
国家科技支撑计划项目(No.2006BAD27B01)
2009-05-30
2009-05-30