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目的初步探讨织纹螺体内河豚毒素(TTX)及其衍生物在不同组织中的分布特征。方法以2004年导致福建仙游地区中毒事件的肇事种红带织纹螺(Nassarius succinctus)为研究对象,将其软组织分为食道、肌肉和内脏三部分,运用液相色谱-质谱联用仪进行毒素分析。结果织纹螺的食道、肌肉和内脏组织中均含有TTX、脱水河豚毒素、三脱氧河豚毒素、单脱氧河豚毒素和加氧河豚毒素等成分,以色谱峰面积为准,trideoxy TTX在各组织中所占比例最高,85%以上,TTX次之。三种组织相比,TTX在内脏中所占的比例最高,而三脱氧毒素在肌肉中所占的比例最高。结论织纹螺不同组织中的河豚毒素及其衍生物的含量与组成情况存在一定差异,这可能是织纹螺对河豚毒素的分配与代谢转化过程的反映。
Abstract：Objective To characterize the anatomical distribution pattern of tetrodotoxin(TTX) and its analogs in different tissues of toxic snails.Methods A toxic snail sample of Nassarius succinctus collected from Xianyou,Fujian Province in 2004 was analyzed to find out the anatomical distribution of TTX and its analogues. The soft tissue of the snails was dissected into three parts of gullet,muscle and viscera,which were analyzed with liquid chromatography-mass spectrometry (LC-MS). Results TTX and its analogs,including anhydroTTX,trideoxyTTX,deoxyTTX and oxoTTX,were detected in all the three tissues. Based on the analytical data of peak area, the trideoxy TTX had the highest proportion in all tissues,occupying above 85% of the total toxins. Among the three tissues, viscera had the highest content of TTX, while mussle had the highest content of trideoxy TTX.Conclusion The differences of content and composition of TTX and its analogs among the three tissues suggested that toxin distribution and biotransformation processes could exist in the snails.
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基本信息:
中图分类号:R155.51
引用信息:
[1]李爱峰,于仁成,周名江,等.河豚毒素及其衍生物在织纹螺体内的解剖分布初探[J].卫生研究,2008(04):448-451.
基金信息:
国家自然科学基金资助项目(No30571426); 国家十五科技攻关计划(No2001BA804A20); 973国家重点基础研究项目(No2001CB409704)
2008-07-30
2008-07-30