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目的探讨不同浓度对羟基苯甲酸乙酯(EP)对雄果蝇的生殖毒性。方法收集基本培养基中8 h内新羽化果蝇,随机选取雄蝇放入对照组和EP浓度组(质量分数分别为0.03%、0.07%和0.10%),雌蝇放入对照组,各自培养5 d后合管,每管1对,每组10次重复,统计10 d亲代(F0)和子一代(F1)产卵量及最大产卵量;F1和第二代(F2)羽化量和羽化率,以及卵-幼虫、幼虫-蛹、蛹-成蝇的天数和总羽化天数。结果 EP处理组F0果蝇的产卵量、羽化量及羽化率与对照组相比呈降低趋势,具有极显著性差异(P<0.01),F1总羽化天数也随着EP浓度的增加而延长。高浓度EP组(0.10%)F1产卵量和F2羽化量与对照组相比也具有极显著性差异(P<0.01),F2总羽化天数与对照组相比有所缩短,差异显著(P<0.05),而0.03%和0.07%组均无显著差异。结论 EP对F0雄性果蝇具有一定的生殖毒性,而高浓度EP对F0的影响可持续至子代,对F1果蝇的生殖有一定的促进作用。
Abstract：Objective To investigate the reproductive toxicity of different concentration of ethylparaben( EP) on male Drosophila. Methods New eclosion flies within 8h in the basal medium were collected and male Drosophila among them were put into control group and EP treating groups with different concentration( 0. 03%,0. 07%and 0. 10%) by random selection. Female Drosophila was all put into control group. After cultured for 5 days by one pair in one tube and 10 times of repetition for each group,the egg laying amount and maximum egg laying amount of 10d parental generation( F0) and first filial generation( F1),the emergence rate and emergence amount of F1and second finial generation( F2),the duration time of eggs-arvae,larvae-pupa,and pupa-adult flies,and the total duration time of emergence were counted. Results The egg laying amount,emergence rate and emergence amount of EP treated parental Drosophila were significantly lower than those of the control group( P < 0. 01). The total duration time of emergence of F1prolongs with the increase of EP concentration. The egg laying amount of F1and emergence rate of F2in high EP concentration group had remarkable differentcompared to those of control group( P < 0. 01). And the total duration time of F2had also shortened significantly compared to that of control group( P < 0. 05),while there was no remarkable difference in 0. 03% and 0. 07% concentration group. Conclusion EP has a certain reproductive toxicity to F0male Drosophila and EP of high concentration can impact sustainable to offspring which has some facilitation effect on F1Drosophila.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2014.02.021
中图分类号:Q965.8
引用信息:
[1]赵晓俊,李雅娟,顾蔚.对羟基苯甲酸乙酯对雄果蝇的生殖毒性[J].卫生研究,2014,43(02):265-270.DOI:10.19813/j.cnki.weishengyanjiu.2014.02.021.
基金信息:
陕西省自然科学基金(No.2012JQ3012); 陕西师范大学研究生培养创新基金(No.2013CXS019)
2014-03-30
2014-03-30