卫生研究

目的观察槲皮素对二氧化硅粉尘所致肺纤维化的影响。方法选取48只雄性健康成年SPF级SD大鼠为研究对象,随机分为6组(对照组,槲皮素干预组,染尘7天、14天、21天和28天组)。对照组大鼠经气管注入无菌生理盐水1 ml,染尘组和槲皮素干预组大鼠注入50 mg/ml二氧化硅混悬液1 ml,预防组以50 mg/kg的槲皮素每天灌胃干预治疗,取肺组织进行HE染色,确定肺泡炎和肺纤维化程度,并检测肺组织中羟脯氨酸(HYP)含量以及过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果对照组大鼠肺组织结构基本正常;染尘组大鼠第7天时肺泡腔内可见大量炎性细胞浸润;14天时肺泡间隔内成纤维细胞显著增多,肺泡结构破坏;21天时大量巨噬细胞聚集形成细胞性结节且结节内有少量的胶原纤维;28天时肺纤维化程度进一步加重,形成广泛纤维化。随染毒时间的增加,肺组织中HYP的表达量逐渐升高,CAT及GSH-Px的活性逐渐降低;加入槲皮素后肺组织中HYP的表达量降低,CAT及GSH-Px的活性升高,差异具有显著性(P<0.05)。结论槲皮素对矽尘所致的肺纤维化有一定的预防作用。

Objective To observe the intervention effect of quercetin to silica dust cause pulmonary fibrosis. Methods Forty-eight healthy male adult SPF SD rats were selected and they were randomly divided into six groups( control group,7 d,14 d,21 d and 28 d dust group,preventive group). Rats in the control group were administrated 1 ml saline via trachea injection. Rats in dust group and preventive group were give silica solution for 1 ml at dose of 50 mg /ml,and the prevention group with quercetin of 50 mg /kg every day lavage treatment intervention. In building on days 7,14,21,28 later,the lung tissue were removaled to HE staining for determining the degree of alveolitis and pulmonary fibrosis. The content of hydroxyproline( HYP) and the activities of catalase( CAT) and glutathione peroxidase( GSH-Px) were detected by using the kits. Results Alveolar septal edema,inflammatory cell infiltration and fibrosis were not found in control group. In the preventive group,lots of inflammatory cells infiltration were observed on days 7. Inflammatory cells were reduced,the number of the fibroblasts and matrix in alveolar septum were obviously increased,and alveolar structure was damaged on day 14.Pulmonary fibrosis was increased,severe fibrosis was found on day 28. Silicon dust after infected lung tissue expression of HYP content increased,the activity of CAT and GSH-Px decrease gradually. After joining quercetin the expression of HYP content gradually reduce,CAT and GSH-Px activity increased,the difference was statistically significant( P <0.05). Conclusion Quercetin of silica dust caused by pulmonary fibrosis have certain prevention.