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目的采用"EpiSkin"3D人工皮肤模型,对20种化学品的刺激性进行评价。方法 20种化学品(固体10 mg、液体10μl)局部接触检测模型表面15 min,用MTT方法测定细胞活力。结果以相对细胞活力值为判定刺激性的依据,在20种化学品中,检测模型特异性为70%,灵敏度为90%。结论 "EpiSkin"3D人工皮肤模型能正确分类所测试的化学品,可以尝试用于化学品皮肤刺激性测试。
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2014.01.039
中图分类号:R16
引用信息:
[1]马丹,赵晓娟,付溥博,等.3D人工皮肤模型用于化学品皮肤刺激性实验的评估[J].卫生研究,2014,43(01):149-151.DOI:10.19813/j.cnki.weishengyanjiu.2014.01.039.
基金信息:
国家质量监督检验检疫总局科研项目(No.2011IK187)
2013-04-08
2013
2013-05-16
2013-05-30
2013
1
2014-01-30
2014-01-30