| 81 | 15 | 51 |
| 下载次数 | 被引频次 | 阅读次数 |
为研究在克山病病区粮食中补充蛋氨酸对大鼠组织硒和谷胱甘肽过氧化物酶 (GPX)活性的影响 ,用克山病病区生产的低硒粮食为主配成低硒基础饲料 ,其硒含量为 0 .0 0 7mg/kg。在此基础上添加不同量的硒蛋氨酸 ,使饲料硒水平分别达到 0 .0 0 7、0 .0 6和 0 .5 0 mg/kg。每一硒水平又分别补充或不补充 4g/kg DL -蛋氨酸 ,配制成含不同硒和蛋氨酸的 6种饲料 ,分别喂养雄性 Wistar断乳大鼠 8周。结果在饲料硒水平为0 .0 0 7mg/kg时 ,补充蛋氨酸组动物除肌肉硒含量低于未补充组外 ,其它组织硒含量和各组织 GPX活力与不补充蛋氨酸动物无显著差异 ;在饲料硒水平为 0 .0 6 mg/kg时 ,补充蛋氨酸组动物组织中的硒含量出现了重新分布 ,最明显的是补充蛋氨酸组动物肌肉的硒含量减少 ,而肝脏和血硒含量增加 ,且各组织中 GPX活力显著大于未补充蛋氨酸组的动物 ;在饲料硒水平为 0 .5 0 mg/kg时 ,补充蛋氨酸组动物组织中硒含量有不同程度下降 ,但 GPX活力仍保持不变。研究结果认为病区粮食中蛋氨酸不足时 ,机体首先利用膳食中的硒蛋氨酸(谷类食物中硒的主要形式 )以替代蛋氨酸参与组织蛋白质的合成。补充蛋氨酸后 ,硒蛋氨酸即可发挥其应有生理功能。进一步提示病区粮食中蛋氨酸不足可能是与克山病发病有关的另一因素。
Abstract：In order to study the effect of dietary methionine on tissue selenium and glutathione peroxidase activity(GPX) in rats fed on grains from Keshan Disease endemic area, a nonpurified selenium(Se) deficient diet(containing Se 0 007mg/kg diet)taken from Keshan Disease endemic area was supplemented with selenomethionine (SeMet) to provide selenium content in diet for 0 007,0 06 and 0 50mg/kg and added or not added with DL methionine (Met) 4g/kg to make 6 kinds of diet. Fifty four male weanling Wistar rats were randomly divided into 6 groups, consuming each one of the 6 diets for 8 weeks. The Se content and GPX activity in tissues of animals with Met supplementation were compared with those without Met supplementation. The results showed that adding Met did not result in significant changes of tissue Se content and GPX activity in animals having dietary Se of 0 007mg/kg, except for lower muscle Se content. However, in animals having dietary Se of 0 06mg/kg and supplementing Met resulted in selenium redistribution in tissues decrease of Se content in muscle, increase of Se content in liver and blood and significant elevation of GPX activity in all tissues. In animals having methionine added and dietary Se of 0 50mg/kg, tissue Se content declined to various extent, while GPX activity remained unchanged. The results suggest that SeMet(main chemical form of Se in cereals) is preferentially incorporated into body protein when dietary methionine is limited. Once Met is supplemented, dietary SeMet would provide more Se for the syntheses of GPX and other selenoproteins. The results further suggest that marginal deficiency in sulfur contaning amino acids in the diet from Keshan Disease endemic area might be an additional factor for the development of Keshan Disease under selenium deficiency.
1 康保安 .改善膳食对预防克山病的效果及其意义 .见 :中国克山病及其预防研究 .北京 :中国环境科学出版社 ,1987,2 88— 2 95
2 周瑞华 ,孙淑庄 ,翟凤英 ,等 .膳食蛋白质水平对大鼠血及组织中硒含量及谷胱甘肽过氧化物酶活力的影响 .营养学报 ,1983,5(2 ) :137— 14 3
3 顾履珍 ,荫士安 ,周瑞华 ,等 .蛋白质水平对喂低硒饲料小鸡渗出性质素质发病率的影响 .营养学报 ,1987,9(1) :8— 13
4 Luo XM,Wei HJ,Yang CL,et al. Selenium intake andmetabolie balance of10 men from a low selenium area of China.Am JClin Nutr,1985 ,42 :31— 37
5 Waschulewski IN,Sunde RA. Effectof dietary methionine on u-tilization of tissue selenium from dietary selenomethionine forglutathione peroxidase in the rat.JNutr,1988,118:36 7— 374
6 Bulter JA,Beilstein MA,Whanger PD. Influence of dietarymathionine on the metabolism of selenomethionine in rats. JNu-tr,1989,119:10 0 1— 10 0 9
7 王光亚 ,周瑞华 ,孙淑庄 ,等 .发、血、尿和其它组织中痕量硒的测定 .营养学报 ,1985 ,7(1) :39— 45
8 VE GB12 388— 909 Xia YM,Hill KE,Burk RF. Biochemical studies of a seleniumdeficient population in China:measurement of selenium ,glu-tathione proxidase and other oxiadant defense index in blood. JNutr,1989,119:1318— 132 6
10 顾履珍 ,许洁 ,周燕 ,等 .克山病区和非病区居民蛋白质营养状况与抗氧化能力的对比调查 .卫生研究 ,1998,2 7(2 ) :14 0— 14 2
11 Seligon FH,Rotruck JT. Tissue sulfhydryl content in rats feddiffecent levels of L-methionine. Fed Proc,1979,38:38212 Meiser A. On the antioxidant effects of asorbic acid and glu-tathione. Biochem Pharmacol,1992 ,44:190 5— 1915
13 杨光圻 ,周瑞华 ,荫士安 ,等 .我国人民硒需要量研究一 .生理需要量 :最低需要量和最低供给量标准 .卫生研究 ,1985 ,14 (5 ) :2 4—2 8
814 杨晓光 ,田园 ,哈鹏程 ,等 .粮食、人血中硒蛋氨酸含量测定 .卫生研究 ,1997,2 6 (2 ) :113— 116
基本信息:
中图分类号:R542.3
引用信息:
[1]田园,曲宁,周燕,许洁,李卫东,夏弈明,顾履珍.克山病病区粮食中补充蛋氨酸对大鼠膳食硒生物利用的影响[J].卫生研究,2001(01):55-58.
基金信息:
:“八五”攻关课题
2001-01-20
2001-01-20