卫生研究

目的观察高糖高脂饲料对Wistar大鼠生长及糖脂代谢的影响。方法雄性Wistar大鼠20只,按体重和空腹血糖随机分为2组(n=10):对照组和实验组,分别以普通饲料和高糖高脂饲料喂养6周,观察大鼠的生长曲线、食物利用率,测定大鼠的糖脂代谢指标。结果喂养6周后,实验组大鼠的体重、食物利用率、内脏脂肪占体重百分比、血清总胆固醇和低密度脂蛋白胆固醇均显著高于对照组(P<0.05),血清高密度脂蛋白胆固醇显著低于对照组(P<0.01)。两组的空腹血糖(FPG)无显著差异。实验组大鼠的糖耐量降低,葡萄糖曲线下面积显著高于对照组。结论正常血糖状态下,高糖高脂饲料可引起Wistar大鼠中心型积聚的体脂增加,血清总胆固醇增加,导致葡萄糖耐量能力减退,为2型糖尿病进程中的重要危险因素。

Objective To study the effects of high-sugar and high-fat diet on the growth and carbohydrate,lipid metabolism of Wistar rats.Methods According to their weight and fasting blood glucose,twenty male Wistar rats were randomly divided into 2 groups(n=10):control group and experimental group,and were fed by normal diet and high-sugar and high-fat diet respectively for 6 weeks.The growth curve of rats and food utilization were observed,the parameters of carbohydrate and lipid metabolism were measured.Results After 6 weeks feeding,the body weight,food utilization,body weight accounted for the percentage of visceral fat,serum total cholesterol and low-density lipoprotein cholesterol of experimental group were significantly higher than those of control group(P<0.05),and serum high-density lipoprotein cholesterol of experimental group was significantly lower than that of control group(P<0.01).The fasting plasma glucose(FPG)of two groups was no significant difference.The glucose tolerance of experimental rats was impaired,the area under the curve of glucose was significantly higher than that of control rats.Conclusions High-sugar and high-fat diet could cause the enhance of central accumulation of body fat,and increased serum total cholesterol,resulting in decreased glucose tolerance ability for type 2 diabetes in the process of an important risk factor.