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目的研究汉族职业噪声接触人群中代谢型谷氨酸受体7基因(metabolic glutamate receptor 7 gene,GRM7)多态性与噪声性听力损失(noiseinduced hearing loss,NIHL)易感性关系。方法采用1比1匹配的巢式病例-对照研究方法,从某钢铁厂噪声接触工人的研究队列中选取听力损失组277例,根据同性别、同工种、年龄相差≤5岁、接触噪声工龄相差≤2年的匹配标准,得到对照组277例。通过中高通量单核苷酸多态性(single nucleotide polymorphisms,SNP)分型检测技术(SNPscanTM法)检测GRM7基因rs3749380、rs11928865、rs9877154、rs3828472、rs9819783、rs11920109、rs1485175及rs9826579位点的多态性。构建加性、显性、隐性、共显性4种基因模型,分析其与NIHL易感性之间的生物学关联。使用Phase 2.0.2构建研究对象可能的双体型,并分析其与NIHL之间的关系。按照累积噪声暴露量(cumulative noise exposure,CNE)分层分析影响因素间交互作用。采用单因素及多因素条件Logistic回归分析GRM7基因与NIHL间的联系。结果听力损失组和对照组一般人口学特征比较发现,吸烟的个体发生NIHL的危险度是不吸烟个体的2.051倍(95%CI 1.4562.891,P<0.001)。GRM7基因多态性与NIHL易感性关系的分析中未发现差异具有统计学意义的结果。结论吸烟可增加职业噪声接触工人发生NIHL的风险;未发现GRM7基因多态性与NIHL易感性之间的关系。
Abstract：Objective To investigate the relationship between metabolic glutamate receptor 7 gene( GRM7) polymorphisms and the susceptibility to noise-induced hearing loss( NIHL) in Chinese Han occupational population. Methods Using a nested case-control study with a 1 ∶ 1 matching,a total of 277 cases of contacting noise workers were selected from a cohort in a steel factory. According to the matching criteria: the same sex,the same type of work,the age difference ≤ 5 years,contact noise length ≤ 2 years,277 controls were selected. The 8 single nucleotide polymorphisms( SNPs) of the GRM7 gene, rs3749380, rs11928865, rs9877154, rs3828472, rs9819783,rs11920109,rs1485175 and rs9826579,were detected by SNPscanTMmethod. The 4 gene models,the additive model,dominant model,recessive model,codominant model,were constructed to explore the biological association with NIHL susceptibility. The possible diplotype of each subject was constructed using Phase 2. 0. 2 to analyze its relationship with NIHL. According to the layered cumulative noise exposure( CNE),the interactions between the influencing factor were analyzed. The relationship between the GRM7 gene and NIHL was analyzed by single factor and multivariate factors conditional logistic regression analysis. Results The comparison of general demographic characteristics between the hearing loss group and control group showed that smoking could increase the risk of developing NIHL by 2. 051 times( 95 % CI 1. 456-2. 891,P< 0. 001). No statistically significant difference was found in the analysis of GRM7 polymorphisms and the susceptibility to NIHL. Conclusion Smoking may increase the risk of NIHL. The relationship between GRM7 polymorphisms and the susceptibility to NIHL has not been found in this study.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2018.02.009
中图分类号:R135.8
引用信息:
[1]于培培,焦洁,谷桂珍,等.GRM7基因多态性与噪声性听力损失易感性的关系[J].卫生研究,2018,47(02):218-227.DOI:10.19813/j.cnki.weishengyanjiu.2018.02.009.
基金信息:
国家自然科学基金(No.81372940); 国家科技支撑计划项目(No.2014BAI12B03)
2017-03-06
2017
2017-09-24
2018-04-10
2018
2
2018-03-30
2018-03-30