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H2AX是组蛋白的一个种类,普遍存在于整个基因组中。据报道,DNA双链断裂后可诱导位于丝氨酸-139位C-端保守区域内的H2AX磷酸化形成γ-H2AX,在荧光显微镜下形成可见的γ-H2AX焦点。用特异性抗体的免疫荧光法检测γ-H2AX已成为测定DSB的金标准。目前,多种物理性、化学性和生物性的因素都能诱导形成γ-H2AX焦点。本文综述了不同因素诱导形成γ-H2AX的机制及其与DNA双链断裂之间的关系的研究进展。
Abstract：H2AX may be one family of histone and distributes among the genome. It is reported that DNA double-strand breaks (DSB) could induce phosphorylation of H2AX at conservative C-terminal region of serine 139 and the formation of γ-H2AX. The γ-H2AX foci can be observed by the fluorescence microscope. An immunofluorescence assay with specific antibodies recognizing γ-H2AX could become the gold standard for detecting the DSB. It was indicated that some of physical, chemical, and biological factors could induce the formation of the γ-H2AX foci. In this paper, the advance of the studies of mechanism of γ-H2AX formation and the association between γ-H2AX and DSB were reviewed.
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基本信息:
中图分类号:Q355
引用信息:
[1]宾萍,郑玉新.γ-H2AX与DNA双链断裂关系的研究进展[J].卫生研究,2007(04):520-522.
基金信息:
国家973计划资助项目(No.2002CB512903); 国家自然科学基金资助项目(No.30400348)
2007-07-30
2007-07-30