卫生研究

目的比较分析两种不同高脂饲料对大鼠血脂的影响,为建立合理而实用的高脂血症动物模型提供依据。方法 Wistar大鼠30只,按体重随机分为对照组(喂饲基础饲料),高脂配方1组和高脂配方2组,每组各10只,实验期间每周记录一次大鼠体重,每两周检测一次血脂,实验周期为6周。结果高脂饲料诱导6周后,高脂配方1组大鼠的血清总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)分别比对照组升高了1.4倍和2.2倍(P<0.01),血清高密度脂蛋白胆固醇(HDL-C)水平比对照组降低了22.2%(P<0.01),血清甘油三酯(TG)水平与对照组无显著差异(P>0.05);高脂配方2组大鼠的血清TC和LDL-C分别比对照组升高了11.5倍和5.7倍(P<0.01),血清TG和HDL-C水平分别比对照组降低了39.7%和34.6%(P<0.01)。结论两种高脂饲料诱导均可建立大鼠高胆固醇血症模型,但均不能显著升高大鼠血清TG水平。

Objective To provide a basis for establishing better animal models with hyperlipidemia by comparing the effects of two different high-fat diets on blood lipids of rats.Methods 30 male Wistar rats were randomly divided into 3 groups(10 rats in each group),including normal control group(NC),high fat formula diet groupⅠ(HFFDⅠ) and high fat formula diet groupⅡ(HFFDⅡ).NC group was fed a stock rodent diet,the HFFD Ⅰ and HFFDⅡ were fed with different high-fat formulae diets.Animals were weighted weekly and blood lipids were determined every two weeks.The experiment lasted for 6 weeks.Results By the end of the experiment,serum total cholesterol(TC) and low-density lipoprotein cholesterol(LDL-C) in HFFDⅠgroup were 1.4 and 2.2 times higher than those of the NC group(P < 0.01).Serum high-density lipoprotein cholesterol(HDL-C) was 22.2% lower than that of NC group.Serum triglyceride(TG) was not significantly different between HFFDⅠand NC group(P > 0.05).Serum TC and LDL-C of HFFDⅡ group were 11.5 and 5.7 times respectively higher than those of the NC group(P < 0.01).Serum TG and HDL-C were 39.7% and 34.6% respectively lower than those of the NC group.Conclusion Animal models with hypercholesteremia can be successfully established by the two methods,but the serum TG level of the rats can not be significantly elevated.