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目的 在中国体重正常成人中比较血脂异常者与血脂正常者肠道菌群的差异。方法 选取参加2018年“中国健康与营养调查”项目的18～59岁体重正常成人作为研究对象，采集空腹静脉血测量甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇及总胆固醇，采集粪便进行16S rRNA基因测序。比较血脂正常组与不同血脂异常亚型组肠道菌群α多样性和β多样性差异，利用线性判别分析效应大小方法进行降维后，通过Logistic回归筛选差异菌属。结果 共纳入1645人，年龄(44.4±9.8)岁。与甘油三酯正常组相比，甘油三酯异常组的α多样性显著降低，β多样性也显著不同，但总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇异常组与正常组组间均未发现α多样性与β多样性的差异。调整年龄、性别、膳食质量、身体活动和地域后，[Ruminococcus]＿gnavus＿group与较高的甘油三酯异常风险相关(OR=1.043,95%CI 1.011～1.077),Faecalibacterium、Collinsella、Clostridia＿UCG-014、Anaerostipes、RF39、Fusicatenibacter等菌属与较低的甘油三酯异常风险相关(OR值分别为0.935、0.965、0.942、0.931、0.964和0.959,95%CI分别为0.893～0.982、0.937～0.994、0.914～0.970、0.886～0.980、0.934～0.995和0.925～0.996)。结论中国体重正常高甘油三酯者的肠道菌群的多样性与特征菌属与甘油三酯正常者显著不同。
Abstract：OBJECTIVE To compare differences in gut microbiota between dyslipidemic and normolipidemic normal-weight adults in China.METHODS Normal-weight adults aged 18-59 years participating in the 2018 “China Health and Nutrition Survey” were enrolled. Fasting venous blood was collected to measure triglycerides(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), and total cholesterol(TC). Fecal samples underwent 16S rRNA gene sequencing. Differences in gut microbial α-diversity and β-diversity were compared between normolipidemic and different dyslipidemic subgroups. Linear discriminant analysis effect size(LEfSe) was used for dimensionality reduction, followed by logistic regression to identify differential bacterial genera.RESULTS A total of 1645 participants were included, with an average age of(44.4 ± 9.8) years. Compared to the normal TG group, the dyslipidemic TG group exhibited significantly reduced α-diversity and distinct β-diversity. No significant differences in α-diversity or β-diversity were found between normal lipidemic groups and dyslipidemic groups for TC, HDL-C or LDL-C. After adjusting for age, sex, diet quality, physical activity, and region, the [Ruminococcus]＿gnavus＿group was associated with a higher risk of dyslipidemic TG(OR=1.043, 95%CI 1.011-1.077). Conversely, the genera Faecalibacterium, Collinsella, Clostridia＿UCG-014, Anaerostipes, RF39, and Fusicatenibacter were associated with a lower risk of dyslipidemic TG(OR=0.935, 95%CI 0.893-0.982; OR=0.965, 95%CI 0.937-0.994; OR=0.942, 95%CI 0.914-0.970; OR=0.931, 95%CI 0.886-0.980; OR=0.964, 95%CI 0.934-0.995; OR=0.959, 95%CI 0.925-0.996, respectively).CONCLUSION The diversity and characteristic bacterial genera of the gut microbiota significantly differ between normal-weight Chinese individuals with hypertriglyceridemia and those with normal TG levels.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2025.05.002
中图分类号:R589.2
引用信息:
[1]黄绯绯,王惠君,关方旭,等.2018年中国十五省(自治区、直辖市)18～59岁体重正常成人肠道菌群与血脂的关联[J].卫生研究,2025,54(05):715-721.DOI:10.19813/j.cnki.weishengyanjiu.2025.05.002.
基金信息:
国家重点研发计划(No.2021YFE0114200)