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目的 对美国国家生物技术信息中心(National Center for Biotechnology Information, NCBI)基因组数据库中干酪乳酪杆菌(Lacticaseibacillus casei)的分类信息进行验证评估，为后续干酪乳酪杆菌种特异序列分析提供准确基因组信息。方法基于NCBI数据库中收录的45个干酪乳酪杆菌基因组信息，分别利用16S rRNA基因和核心基因序列开展系统发育分析，利用全基因组序列开展平均核苷酸一致性(average nucleotide identity, ANI)分析。结果 4个基因组未能提取到16S rRNA基因序列。其他41个基因组的ANI和核心基因系统发育分析结果均高度一致，21个(51.22%)为干酪乳酪杆菌，20个(48.78%)为干酪乳酪杆菌近缘菌种。16S rRNA基因序列无法在种水平上准确鉴定干酪乳酪杆菌及其近缘菌种。结论 基于NCBI基因组数据库中收录的干酪乳酪杆菌基因组开展相关研究时，需对菌株的分类信息进行准确界定。
Abstract：OBJECTIVE Reverificate the taxonomic information of Lacticaseibacillus casei in the genome database of National Center for Biotechnology Information(NCBI) to afford accurate genomic information for L. casei species-specific sequence analysis.METHODS Based on the whole genome sequence downloaded from NCBI database, the taxonomic information of 45 L. casei was reverificated by average nucleotide identity(ANI) analysis and phylogenetic analysis based on 16S rRNA gene and 49 core genes.RESULTS No 16S rRNA gene sequence was extracted from 4 genomes. Of the remaining 41 genomes, the analysis results of ANI were highly in accordance with that of core gene phylogenetic analysis. The results were 21 genomes identified as L. casei and 20 genomes as L. casei related species. Using 16S rRNA gene alone could not accurately identify L. casei and its related species.CONCLUSION It is necessary to reverificate the taxonomic information of L. casei in NCBI genome database before using its genome information for further analysis.
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基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2026.04.005
中图分类号:TS201.3
引用信息:
[1]汪琦,杨立峰,王伟,等.基于全基因组序列评估美国国家生物技术信息中心数据库中干酪乳酪杆菌分类准确性[J].卫生研究,2026,55(04):558-566.DOI:10.19813/j.cnki.weishengyanjiu.2026.04.005.
基金信息:
食品安全国家标准制定项目(No.spaq-2025-36); 海关总署科研项目(No.2024HK126)
2026-07-15
2026-07-15