卫生研究

目的测定食物中叶黄素(Lut)、隐黄素(Cryp)、α-胡萝卜素(α-C)、β-胡萝卜素(β-C)和番茄红素(Lyco)的水平。方法采用高效液相色谱法(HPLC)。HPLC色谱柱:Develosil C30柱;流动相:A(甲醇∶叔丁基甲基醚∶水=83∶15∶2),B(甲醇∶叔丁基甲基醚∶水=8∶90∶2),梯度洗脱;检测器:紫外-可见光检测器;检测波长:450nm。结果Lut在0.013⁰.508μg/ml范围内与峰面积的线性相关,R2=0.999,加标回收率为105.9%,RSD=4.62%;Cryp在0.015⁰.605μg/ml范围内与峰面积的线性相关,R2=1.000,加标回收率为94.7%,RSD=3.50%;α-C在0.011⁰.432μg/ml范围内与峰面积的线性相关,R2=1.000,加标回收率为95.8%,RSD=3.83%;、β-C在0.013⁰.518μg/ml范围内与峰面积的线性相关,R2=1.000,加标回收率为108.1%,RSD=2.97%;Lyco在0.012⁰.498μg/ml范围内与峰面积的线性相关,R2=1.000,加标回收率为104.5%,RSD=4.24%。结论该方法同时测定了蔬菜中的Lut、Cryp、α-C、β-C和Lyco5种类胡萝卜素组分,方法简便、快速、准确。

ObjectiveTo detect α-carotene(α-C),β-carotene(β-C),cryptoxanthin(Cryp),lutein(Lut)and lycopene(Lyco)levels in vegetables.Methods A HPLC method with C30 column was used to detect several carotenoids at the wave length of 450nm.A gradient elution method with methanol,methyl-t-butyl-ether and ammonium acetate solution was used to separate the carotenoids.Results The level of Lut,Cryp,α-c,β-c and lycopene were linearly related with the peak area.The recoveries of the standard were between 94.7%-108.1%.The relative standard deviations were less than 4.62%.Conclusion The method could be simple and credible.It could separate 5 carotenoids in vegetables simultaneously.