| 442 | 2 | 60 |
| 下载次数 | 被引频次 | 阅读次数 |
目的 研究SEC16B基因rs633715、DNAJC27基因rs713586、FTO基因rs11642015和MC4R基因rs6567160多态性与汉族学龄前儿童超重肥胖的关联性。方法 选取2022年来自“早期儿童营养包干预长期营养健康作用评估”项目监测点的河南省和贵州省749名2～6岁汉族儿童为研究对象，分成超重肥胖组和正常对照组。采用KASP SNP分型技术对rs633715、rs713586、rs11642015和rs6567160进行基因多态性检测。使用χ2检验分析4个位点的基因型多态性分布，使用多因素Logistic回归模型分析4个位点与学龄前儿童超重肥胖的关联性。结果 rs633715和rs6567160的基因型多态性在河南省和贵州省学龄前儿童中的分布有统计学意义(P<0.05)。rs633715位点的CC纯合突变型和隐性模型与学龄前儿童患超重肥胖的风险有关[OR及95%CI分别为2.915(1.163～7.305)和2.997(1.226～7.323),P<0.05];rs713586位点的TC杂合突变型和显性模型与学龄前儿童患超重肥胖的风险有关[OR及95%CI分别为2.362(1.054～5.289)和2.362(1.054～5.289),P<0.05]。rs11642015和rs6567160位点的多态性与学龄前儿童超重肥胖的关联无统计学意义。rs633715和rs713586的累积效应分析结果显示，携带风险基因型的数量与学龄前儿童患超重肥胖的风险存在正向关联(P趋势<0.01)。结论 在汉族学龄前儿童中，SEC16B基因rs633715和DNAJC27基因rs713586位点的多态性与超重肥胖易感性有关，且SEC16B基因rs633715和DNAJC27基因rs713586位点的多态性对学龄前儿童超重肥胖易感性有累积效应，携带风险基因型的数量与儿童超重肥胖风险呈正向关联。
Abstract：OBJECTIVE To investigate the association of polymorphisms in SEC16B rs633715, DNAJC27 rs713586, FTO rs11642015 and MC4R rs6567160 with overweight and obesity in Han Chinese preschool children.METHODS A total of 749 Han Chinese preschool children from Henan and Guizhou Province of Long-term Health Effects Assessment Project of Infants and Toddlers Nutritional Pack were selected for the study and divided into an overweight and obese group and a normal control group in 2022. rs633715, rs713586, rs11642015 and rs6567160 were genotyped using Kompetitive allele-specific PCR(KASP) technology. The distribution of genotypic polymorphisms was compared using the χ2 test. The association between the four loci and overweight and obesity in preschool children was analyzed using a multifactorial logistic regression model.RESULTS The statistical analysis revealed a significant disparity(P<0.05) in the distribution of genotypic polymorphisms of rs633715 and rs6567160 among preschoolers in Henan and Guizhou Province. CC heterozygous mutant and recessive models at rs633715 locus were associated with susceptibility to overweight and obesity in preschool children [OR and 95% CI 2.915(1.163-7.305), and 2.997(1.226-7.323), respectively, both P<0.05]. TC heterozygous mutant and dominant models at rs713586 locus were also associated susceptibility to overweight and obesity in preschool children(OR and 95% CI were 2.362(1.054-5.289)and 2.362(1.054-5.289), respectively, both P<0.05). rs11642015 and rs6567160 loci were not associated with susceptibility to overweight and obesity in preschool children(P>0.05). The result of the analysis of the cumulative effect of rs633715 and rs713586 showed that the number of genotypes carrying the risk genotype was positively associated with the risk of overweight and obesity in preschool children(Ptrend<0.01).CONCLUSION Among Han Chinese preschool children, SEC16B rs633715 and DNAJC27 rs713586 were associated with susceptibility to overweight and obesity in preschool children. Moreover, rs633715 and rs713586 had a cumulative effect on susceptibility to overweight and obesity in preschool children, the number of risk genotypes carried was positively associated with childhood overweight and obesity risk.
[1] (NCD-RISC) NRFC.Worldwide trends in body-mass index,underweight,overweight,and obesity from 1975 to 2016:a pooled analysis of 2416 population-based measurement studies in 1289 million children,adolescents,and adults[J].Lancet,2017,390(10113):2627-2642.
[2] 国家卫生健康委疾病预防控制局.中国居民营养与慢性病状况报告(2020年)[M].北京：人民卫生出版社，2021:42-45.
[3] VERDUCI E,DI PROFIO E,FIORE G,et al.Integrated approaches to combatting childhood obesity[J].Ann Nutr Metab,2022,78(Suppl 2):8-19.
[4] LIU D,HAO Y X,ZHAO T Z,et al.Childhood BMI and adult obesity in a Chinese sample:a 13-year follow-up study[J].Biomed Environ Sci,2019,32(3):162-168.
[5] SHUNGIN D,WINKLER T W,CROTEAU-CHONKA D C,et al.New genetic loci link adipose and insulin biology to body fat distribution[J].Nature,2015,518(7538):187-196.
[6] 付利万，张美仙，高利旺，等.SEC16B基因多态性与体质指数和肥胖关联的Meta分析[J].中华流行病学杂志，2016,37(9):1288-1295.
[7] MEI H,YIN B,YANG W,et al.Associations between gene-gene interaction and overweight/obesity of 12-month-old Chinese infants[J].Biomed Res Int,2022,2022:1499454.
[8] DASTGHEIB S A,BAHRAMI R,SETAYESH S,et al.Evidence from a meta-analysis for association of MC4R rs17782313 and FTO rs9939609 polymorphisms with susceptibility to obesity in children[J].Diabetes Metab Syndr,2021,15(5):102234.
[9] FRAYLING T M,TIMPSON N J,WEEDON M N,et al.A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity[J].Science,2007,316(5826):889-894.
[10] SCUTERI A,SANNA S,CHEN W M,et al.Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits[J].PLoS Genet,2007,3(7):e115.
[11] YU K,LI L,ZHANG L,et al.Association between MC4R rs17782313 genotype and obesity:a meta-analysis[J].Gene,2020,733:144372.
[12] AKIYAMA M,OKADA Y,KANAI M,et al.Genome-wide association study identifies 112 new loci for body mass index in the Japanese population[J].Nat Genet,2017,49(10):1458-1467.
[13] 霍军生.营养包：从科学研究到贫困地区婴幼儿营养干预[J].卫生研究，2021,50(3):357-359.
[14] WHO Child Growth Standards based on length/height,weight and age[J].Acta Paediatr Suppl,2006,450:76-85.
[15] DE ONIS M,ONYANGO A W,BORGHI E,et al.Development of a WHO growth reference for school-aged children and adolescents[J].Bull World Health Organ,2007,85(9):660-667.
[16] ELKS C E,DEN HOED M,ZHAO J H,et al.Variability in the heritability of body mass index:a systematic review and meta-regression[J].Front Endocrinol,2012,3:29.
[17] LOCKE A E,KAHALI B,BERNDT S I,et al.Genetic studies of body mass index yield new insights for obesity biology[J].Nature,2015,518(7538):197-206.
[18] SHI R,LU W,TIAN Y,et al.Intestinal SEC16B modulates obesity by regulating chylomicron metabolism[J].Mol Metab,2023,70:101693.
[19] VOGELEZANG S,BRADFIELD J P,AHLUWALIA T S,et al.Novel loci for childhood body mass index and shared heritability with adult cardiometabolic traits[J].PLoS Genet,2020,16(10):e1008718.
[20] LV D,ZHANG D D,WANG H,et al.Genetic variations in SEC16B,MC4R,MAP2K5 and KCTD15 were associated with childhood obesity and interacted with dietary behaviors in Chinese school-age population[J].Gene,2015,560(2):149-155.
[21] CHERIAN P T,AL-KHAIRI I,SRIRAMAN D,et al.Increased circulation and adipose tissue levels of DNAJC27/RBJ in obesity and type 2-diabetes[J].Front Endocrinol,2018,9:423.
[22] PARK S,YANG H J,KIM M J,et al.Interactions between polygenic risk scores,dietary pattern,and menarche age with the obesity risk in a large hospital-based cohort[J].Nutrients,2021,13(11):3772.
[23] SPELIOTES E K,WILLER C J,BERNDT S I,et al.Association analyses of 249,796 individuals reveal 18 new loci associated with body mass index[J].Nat Genet,2010,42(11):937-948.
[24] RESHETNIKOVA Y,CHURNOSOVA M,STEPANOV V,et al.Maternal age at menarche gene polymorphisms are associated with offspring birth weight[J].Life,2023,13(7):1525.
[25] EVENSEN E,WILSGAARD T,FURBERG A S,et al.Tracking of overweight and obesity from early childhood to adolescence in a population-based cohort-the Troms? Study,Fit Futures[J].BMC Pediatr,2016,16:64.
基本信息:
DOI：10.19813/j.cnki.weishengyanjiu.2024.02.009
中图分类号:R723.14
引用信息:
[1]彭锣兰,宫照龙,韩超,等.SEC16B、DNAJC27、FTO和MC4R基因多态性与汉族学龄前儿童超重肥胖的关联性[J].卫生研究,2024,53(02):229-236.DOI:10.19813/j.cnki.weishengyanjiu.2024.02.009.
基金信息:
国家重点研发计划(No.2020YFC2006301); 早期儿童营养包干预长期营养健康作用评估项目(No.131031107000200001)
2024-03-20
2024-03-20